PDX1 is involved in KLF11-induced inhibition of the human insulin promoter in rodent beta-cells
G Päth, D Danassi, N Perakakis, W Zhou, M Alt, S van Loosen, K Laubner, J Seufert · Diabetologie und Stoffwechsel · 2009 · Heft 05
Bibliografische Angaben
Zusammenfassung
KLF11 is a pancreas enriched member of the Sp1/KLF transcription factor family which plays an important role in mammalian gene regulation. Previously we demonstrated that human (h)KLF11) inhibits human insulin promoter (hInsP) activity in rodent beta-cell lines via an unknown mechanism. Here we present results from ongoing experiments in which we test the hypothesis that KLF11 interferes with the major hInsP transactivator PDX1. We performed transient cotransfections of INS-1E and beta-TC3 beta-cells and HEK293 non-beta-cells with hInsP-controlled SEAP (secreted alcaline phosphatase) reporter plasmids and CMV-dr…
