Cloning and functional characterization of the guinea pig trypsinogen

Introduction: Preliminary studies indicate that trypsin regulates the function of guinea pig pancreatic ducts via activation of proteinase-activated receptors. However, there are no data on the number and characteristics of trypsinogen isoforms expressed in the guinea pig pancreas. Aim: Our aim was to purify and clone the trypsinogen isoforms from the guinea pig pancreas and characterize their activation properties. Methods: Ecotin-affinity chromatography was used for the purification of trypsinogen from pancreatic homogenates. Total RNA was isolated with the RNAqueous kit (Ambion) and reverse-transcribed with t…