Differences in HCO3-/CO2 permeabilities at the apical and basolateral membranes of cystic fibrosis human pancreatic duct cells
C Székely, Z Rakonczay Jr, A Fearn, P Hegyi, I Boros, T Takács, J Lonovics, MA Gray, BE Argent · Zeitschrift für Gastroenterologie · 2007 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: Pancreatic duct cells secrete an alkaline, HCO3- rich fluid, and this process is markedly reduced in cystic fibrosis (CF). Investigations of the H+ and HCO3- transporters involved in ductal HCO3- secretion have been performed mainly on animal tissue. The aim of this study was to characterize the HCO3-/CO2 permeabilities and acid/base transporters in polarised CFPAC-1 human pancreatic duct cells (derived from a CF patient). Methods: CFPAC-1 cells grown on Transwells were loaded with the pH sensitive fluorescent dye BCECF, and mounted into a perfusion chamber, which allowed the simultaneous perfusion…
