EGFR-overexpression induces telomerase reactivation and cell cycle progression as distinct steps in a cellular model of oral esophageal carcinogenesis
N Hirt, S Heeg, A Queisser, S Hauß, EM Egenter, H Kunert, M Doebele, M Quante, G Goessel, H Nakagawa, R Beijersbergen, H Blum, O Opitz · Zeitschrift für Gastroenterologie · 2007 · Heft 09
Bibliografische Angaben
Zusammenfassung
Introduction: In our cellular model of oral-esophageal carcinogenesis EGFR-overexpression plays a crucial role in in vitro transformation, telomerase-reactivation and enhanced proliferation of immortalized cyclinD1-overexpressing/p53-inactivated keratinocytes (OKF6-D1/d.n.p53). We previously demonstrated that this is mediated by the activation of the PI3K/AKT-pathway. Our aim was to characterize the cellular mechanisms involved in the telomerase-reactivation and acceleration of the proliferative capacity induced by EGFR-overexpression leading to in vitro transformation. Methods: We overexpressed EGFR in immortal…
