Targeting of P53-transcriptional dysfunction by conditionally replicating adenovirus is not limited by P53-homologues
F Kühnel, E Gürlevik, T Wirth, N Malek, M Müller-Schilling, M Manns, L Zender, S Kubicka · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Bibliografische Angaben
Zusammenfassung
A hallmark of hepatocellular carcinomas is the loss of p53 or its transcriptional functions. In this study we describe the generation of the conditionally-replicating adenovirus Adp53sensor for the treatment of p53-dysfunctional HCC. p53-selective attenuation of viral replication was achieved by using p53-dependent expression of the transcriptional repressor GAL4-KRAB that was directed against the adenoviral E1A locus. Adp53sensor shows efficient replication in p53-dysfunctional, but not in p53-active cells. In p53-dysfunctional cells, p53-analogous transcriptional activity by other p53-family members was not su…
