In vitro comparison of iron oxide contrast agents for labelling of human hepatocytes
D Mücke, N Raschzok, MK Adonopoulou, A Zielinski, N Billecke, W Werner, U Teichgräber, IM Sauer · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Bibliografische Angaben
Zusammenfassung
Aim: Magnetic resonance imaging (MRI) is a promising approach for non-invasive monitoring of liver cell transplantation. Cellular imaging requires labelling with iron oxide contrast agents. Aim of this study was to compare in vitro clinically approved iron oxide particles (SPIO) and micron-sized iron oxide particles (MPIO) for labelling of human hepatocytes. Materials & Methods: Primary human hepatocytes were isolated from liver specimen obtained from 9 different patients. Cells were cultivated in adhesion and incubated with SPIO (Endorem) or MPIO. As control groups, native cells and iron- stimulated cells were…
