Legionella pneumophila induced microRNA expression in human lung epithelial cells

Legionella pneumophila cause severe community- and hospital-acquired pneumonia. Lung airway and alveolar epithelial cells comprise an important sentinel system in airborne infections. However, little is known on specific Legionella-induced signal transduction in the lung, especially the effect on chromatin remodeling and miRNA expression are widely unknown. In this study we performed a comprehensive analysis of miRNAs in L. pneumophila-infected lung epithelial cells by Next Generation Sequencing (Solexa Illumnia) and characterized chromatin remodeling on distinct gene promoters by quantitative Chromatin Immunopr…