Effects of insulin on plasma metabolome and subcutaneous fat tissue transcriptome in humans

Aims: Insulin, the dominant anabolic hormone, suppresses endogenous glucose production, stimulates glucose utilization by insulin-sensitive tissues and inhibits the lipolysis in fat. Few studies describe insulin-dependent transcriptome changes and no data exist for metabolome. In this pilot study we examined acute insulin effects on plasma metabolites and subcutaneous adipose tissue transcriptome under clamped euglycemia and hyperglycemia. Methods: Healthy obese men (n=14) underwent a placebo infusion test (0.9% NaCl-infusion for 4h) and/or a hyperinsulinemic, euglycemic clamp (EC), and/or a hyperinsulinemic, hy…