CareLit Fachartikel

Effects of Genistein on hepatic insulin signaling

S Lünse, A Krüger, C Brühmann, M Glanemann, D Knobeloch · Zeitschrift für Gastroenterologie · 2011 · Heft 01

Dokument
922289
CareLit-ID
Jahr
2011
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
S Lünse, A Krüger, C Brühmann, M Glanemann, D Knobeloch
Ausgabe
Heft 01 / 2011
Seiten
–
Erschienen: 2011-01-13 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0030-1269572

Zusammenfassung

Hepatic steatosis in the context of nonalcoholic fatty liver disease (NAFLD) is associated with insulin resistance. In our study we investigated the effects of genistein on insulin-responsive downstream targets in an in-vitro model of hepatic steatosis. Primary human hepatocytes and HuH-7 cells were treated with oleic and palmitic acid (2: 1 ratio, final concentration 0.5 mM) for 24h to induce an intracellular fat overaccumulation. Oilred O and SRB staining evaluated the in-vitro model of hepatic steatosis. Cells were treated with different concentrations of genistein (7.5 up to 90µM) with increased time (1 up t…

Schlagworte

flavonoids genistein hepatic steatosis insulin resistance nonalcoholic fatty liver disease phytoestrogens Zeitschrift für Gastroenterologie