Single-cell Phospho-Profiling in Pediatric B-cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL) Reveals Signaling Differences in Cytogenetic and Prognostic Subgroups

Reasons for poor response to therapy and/or relapse in pediatric ALL remain poorly understood, especially in patients originally stratified into low-risk groups. As it is widely known that oncogenesis and tumor progression are supported by alterations in cellular signaling, we analyzed 22 primary xenografted BCP- ALL samples in respect to their potential to react to a given stimulus in vitro and related the phosphorylation profiles to the clinical characteristics of the corresponding patients. Phospho-specific antibodies in combination with surface staining in flow cytometry were used to analyze specific signali…