Neuroblastoma develops from neural crest stem cells
S Lindner, JH Schulte, A Bohrer, J Maurer, K DePreter, S Lefever, L Heukamp, J Molenaar, R Versteeg, T Thor, J Vandesompele, F Speleman, H Schorle, A Eggert, A Schramm · Klinische Pädiatrie · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Neuroblastoma (NB) is presumed to arise from neural crest stem cells (NCSCs), but the exact developmental window when tumor cells arise remains to be determined. Methods: The conditionally immortalized multipotent NCSC line, JoMa1, proliferates in the presence of tamoxifen-induced c-Myc (c-MycERT) expression, and differentiates into neural crest derivates upon tamoxifen withdrawal. We stably transfected JoMa1 cells with cDNA encoding NMYC, TrkA, GFP or ALK[F1174L], a mutation found in primary NBs. Proliferation and tumorigenicity were analysed in vitro and as xenogratfs in nude mice. Results: JoMa1-N…
