Fecal supernatants from constipated IBS patients increase colonic permeability in mice by occludin degradation linked to cysteine-protease activity
A Annaházi, R Róka, M Leveque, H Eutamene, L Ferrier, K Gecse, A Rosztóczy, O Inczefi, G Ollé, F Izbéki, T Molnár, T Piche, V Theodorou, T Wittmann, L Bueno · Zeitschrift für Gastroenterologie · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and aims: Increased gut permeability has been described in all IBS subgroups. We have previously shown that fecal cysteine-protease (CP) activity is elevated only in constipated IBS (IBS-C) patients among IBS subtypes. Since CPs are involved in the increase of airway permeability by digesting tight junction proteins, we evaluated the ability of CP in the IBS-C fecal supernatant (FSN) to increase permeability and degrade tight junction proteins in mice and T84 human cell cultures. Methods: CP activity was assayed in fecal samples from healthy subjects (n=20) and IBS-C patients (n=25) using a selective…
