A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

Introduction: Ovarian tissue freezing and autotransplantation is a promising option for preserving fertility of female cancer patients. There are several in vivo, but no established in vitro models to evaluate frozen/thawed human ovarian tissue prior to autotransplantation. Objective: To establish a new in vitro model for endocrine assessment of frozen/thawed human ovarian tissue prior to autotransplantation. Materials & Methods: Ovarian tissues were obtained by laparoscopy from 5 patients 19 to 34 years old recently diagnosed with cancer. 200mg cortical tissue from each patient was used and further divided into…