MicroRNA expression profiles in trophoblastic cells

Ziel: MicroRNAs are small single-stranded RNA molecules playing an important role in the post-transcriptional regulation of gene expression. Aim of this study was to analyze miRNA expression in four trophoblastic cell lines (JEG–3, ACH–3P, AC1-M59, HTR8/SV neo) before and after LIF challenge. Methodik: Cells were stimulated or not with LIF for 4h and total RNA containing microRNAs was isolated. Expression of 762 microRNAs was screened in biological duplicates. HTR–8 cells were karyotyped through Multiplex-Fluorescence In Situ Hybridisation (M-FISH). Furthermore, expression kinetics of miR–9, miR–141, miR–21, miR…