Characterization of liver specific functions of upcyte® hepatocytes grown in 3D and co-cultured with upcyte® endothelial cells
C Dähn, N Hewitt, D Maltman, G Talas, S Przyborski, S Heinz, A Nörenberg, K Scheller, J Braspenning · Zeitschrift für Gastroenterologie · 2012 · Heft 01
Bibliografische Angaben
Zusammenfassung
Objective Major disadvantages of using human primary hepatocytes are their lack of proliferation and dedifferentiation in culture. Therefore, proliferating hepatocytes in combination with sophisticated 3D-culture systems capable of sustaining long-term functional cultures are highly desirable.Medicyte has developed a new technology that drives differentiated primary cells into proliferation in vitro, without uncontrolled cell growth or loss of phenotype. Upcyte® technology is based on targeted genetic modification of human primary cells allowing multiple passaging and the generation of billions of quasi primary…
