Functional analysis of immunological mechanisms following mouse liver transplantation

Aims: Mouse liver transplantation (MLTx) results in spontaneous allospecific tolerance induction; however, the mechanisms orchestrating this phenomenon are not identified. For this study characterizes the magnitude, phenotype, and persistence of alloreactive T cell responses. Methods: Balb/C livers were transplanted into B10 recipients. The alloreactive T-cell response was studied by 1) adoptively transferred transgenic alloreactive T cells, 2) in vivo MLR, for the endogenous alloreactive T cell response, and 3) in vivo cytotoxicity assays. Results: Adoptive transfer of TCR-tg donor-reactive T cells with direct…