Acetylation of Myeloid-Specific Transcription Factor C/EBPα

Previously, we described new mechanism of G-CSF-triggered granulocytic differentiation via activation of the enzyme Nicotinamide Phosphorybosyltransferase (Nampt) leading to NAD+ production and activation of NAD+-dependent protein deacetylases SIRT1 and SIRT2. In order to investigate the mechanism of Nampt-triggered myeloid differentiation, we investigated whether myeloid-specific transcription factor C/EBPα could be acetylated. We found that SIRT1 and SIRT2 bind to and activate C/EBPα. We found that C/EBPα is acetylated on Lys 161 and generated rabbit polyclonal antibody specifically recognised acetyl-Lys 161 C…