Investigation of pancreatic ductal fluid and HCO3-secretion in NHERF1 knockout and wild-type mice
P Pallagi, A Kumar Singh, P Hegyi, V Venglovecz, R Engelhardt, B Riederer, T Takács, T Wittmann, U Seidler, Z Rakonczay Jr · Zeitschrift für Gastroenterologie · 2012 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Na+/H+ exchanger regulatory factor-1 (NHERF-1) is a scaffolding protein which is responsible for the apical localization of cystic fibrosis transmembrane conductance regulator (CFTR), a key player in pancreatic ductal bicarbonate secretion. The aim of this study was to evaluate the role of NHERF-1 in pancreatic ductal localization of CFTR, and in bicarbonate and fluid secretion. Methods: The expression of CFTR was analysed by immunohistochemistry. Pancreatic juice was collected from anesthetized wild-type (WT) and NHERF-1 knock-out (KO) mice in basal and secretin-stimulated conditions. We isolated in…
