Hypoxia-induced changes in expression levels of miR-210, -183 and -744 in human lung fibroblasts

Aims: Micro-RNA (miRNA) profiles of human pulmonary arterial fibroblasts (hPAFb) were measured in order to reveal hypoxia-induced changes in miRNA profiles. Pathway analysis based on miRNA target prediction was compared with pathway analysis based on mRNA microarrays. Methods: Fibroblasts from pulmonary arteries of a healthy human lung were cultured and kept under normoxic and hypoxic (FiO2=0.1) conditions for 24h. Total RNA was isolated using a phenol-/guanidine-based method. Success of the hypoxic treatment was confirmed by measuring the expression of the PGK1 gene by quantitative real-time PCR (qRT-PCR). The…