Transforming growth factor beta 1-stimulated clone (TSC) 22 D4 is a novel key transcriptional co-regulator of hepatic metabolism

Background and aims: The transcriptional co-regulators TBL1/TBLR1 are key metabolic players, controlling liver and serum triglyzeride metabolism. Our aim was to screen for potential novel interaction partners and define their role in metabolism. Methods: Using luminescence-based mammalian Interactome mapping, we screeneed for TBL1/TBLR1 interaction partners. Adenoviruses expressing a TSC22D4 or a non-specific shRNA were generated for genetic knockdown of TSC22D4. For genetic overexpression of TSC22D4 we established adenoviruses expressing TSC22D4 cDNA under the control of the CMV promoter. For long-term knockdow…