HTR8/SVneo, but not JEG3, cells display trophoblast progenitor cell-like characteristics indicative of self-renewal, repopulation activity and expression of “stemness”- associated…

Introduction: JEG3 and HTR8/SVneo cells are popular trophoblastic cell lines for use as models of extravillous trophoblast. Recently, choriocarcinoma is thought to constitute a population of tumor with preserved stem cell properties. Furthermore, a side-population of HTR8/SVneo cells demonstrated self-renewal characteristics. Aim of this study was to correlate expression of various transcription factors associated with “stemness”, cell fate and trophoblast stem cells in these popular cell lines with stem/progenitor cell-like functions e.g. self-renewal and repopulation activity. Methods: JEG3 and HTR8/SVneo cell…