The cytokine TNFα inhibits cortical spreading depolarization (CSD) via the TNF receptor 2 at interneurons in adult rat

Question: Which type of TNF receptors and which cells are the cause for diminished amplitudes of CSD by TNFα in the adult rat? Methods: In spontaneously breathing anesthetized adult rats (sodium thiopentone, 100 mg/kg, i.p.) KCl-induced CSD were recorded in cerebral cortex with two pairs of micropipettes at layer V. A cortical area was pretreated (1 hour) either with a TNF receptor 1 (TNFR1) antibody, or with a TNF receptor 2 (TNFR2) antibody, or with the GABAA receptor antagonist bicuculline. We tested for CSD prior and after pretreatment, and during the subsequent application of 5 ng TNFα for two hours. Effect…