Downregulation of Hemeoxygenase-1 and Altered Cellular Homeostasis in Cystic Fibrosis
SP Chillappagari, S Venkatesan, V Garapati, P Mahavadi, A Munder, A Seubert, A Guenther, B Schmeck, B Tümmler, M Henke · Pneumologie · 2014 · Heft 06
Bibliografische Angaben
Zusammenfassung
Hemeoxygenase-1 (HO-1), an inducible heat shock protein, is upregulated in response to multiple cellular insults via oxidative stress, lipopolysaccharides (LPS), and hypoxia. In this study, we investigated the role of toll-like receptor 4 (TLR4), hypoxia-inducible factor-1α (HIF-1α) and iron on HO-1 expression in cystic fibrosis (CF). Immunohistochemical analysis of TLR4, HO-1, ferritin and HIF-1α were performed on lung sections of CFTR-/- and wildtype mice. CFBE41o- and 16HBE14o- cell lines were employed for in vitro analysis via immunoblotting, immunofluorescence, real-time PCR, luciferase reporter gene analys…
