Generation of a NKX2.1 knockin human induced pluripotent stem cell reporter line for monitoring the generation of respiratory cells

One promising therapeutic option to cure hereditary pulmonary diseases like cystic fibrosis might be a cell replacement therapy comprising the generation of patient specific autologous induced pluripotent stem cells (iPSCs), followed by the correction of the genetic mutation, differentiation into the needed airway cell type and replacement of the endogenous cells. For long term restoration, most likely airway progenitor or stem cells like basal cells or submucosal gland duct stem cells will be required. A prerequisite is the development of an efficient and robust protocol for the generation of the desired airway…