CareLit Fachartikel
A frog-based approach to study pancreatic development
V Henkel, S Kühl, M Kühl, T Seufferlein, A Kleger · Zeitschrift für Gastroenterologie · 2014 · Heft 08
Dokument
932497
CareLit-ID
Jahr
2014
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Pancreatic development is a yet incomplete understood process. This is mainly due to a lack of fast and efficient model systems. While mouse models are time-consuming and cost-intensive, pluripotent stem cell based assays require a complex cocktail of growth factors to drive them to the respective lineage. Herein, we approached this hurdle by using Xenopus laevis as an in vivo model and provide proof of concept by targeting transcription factors of potential relevance for pancreatic development. A series of methods is applied (i) morpholino based UTR-targegting at different embryonic stages (ii) in situ hybridiz…
Schlagworte
Pancreatic
Development
Efficient
Model
Based
Factors
Provide
Study
Incomplete
Understood
Process
Mainly
Zeitschrift für Gastroenterologie
