Liver sinusoidal endothelial cells and Kupffer cells contribute to HCV innate immune response via TLR3
X Cheng, M Boxtermann, B Höchst, JH Bockmann, R Bester, B Holzmann, P Knolle, N Hüser, D Hartmann, K Esser, U Protzer · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
Hepatitis C virus (HCV) infection is a major cause of liver cirrhosis and hepatocellular carcinoma. Within days post infection, innate immunity is activated by HCV reflected by an induction of IFN-stimulated genes (ISGs). The precise cell type and cellular pathogen pattern recognition receptors (PRRs) involved in innate immune sensing of HCV, however, still remain unknown. To study this as close as possible to the physiological situation, we utilized ex vivo perfused human liver tissue. By pulse perfusing the liver with HCV for 1h and chase perfusing the liver with HCV free media for 23h, we found the virus afte…
