Hepatic Proteome and Lipid Profiling of Wild Type and Lipocalin-2-Deficient Mice in Experimental Steatosis

Background: Lipocalin-2 (LCN2) or neutrophil gelatinase-associated lipocalin (NGAL) is a small secreted adipokine belonging to the lipocalin family [1]. It binds and transports small hydrophobic molecules and limits bacterial growth by sequestering iron-containing siderophores. In the liver, LCN2 plays a protective role in inflammation, infection, and cellular stress. Recently, we demonstrated that LCN2 regulates lipid droplet protein Perilipin 5 (PLIN5) expression in primary hepatocytes and showed that LCN2 animals are more prone to hepatic inflammation and steatosis [2 – 4]. Methods: We here comparatively anal…