Different oncogenic potential in the mouse liver of mutant forms of the p110alpha catalytic subunit of phosphoinositide-3-kinase (PIK3CA)
K Annweiler, K Evert, A Cigliano, M Sini, G Latte, M Frau, RM Pascale, F Dombrowski, DF Calvisi, M Evert, K Utpatel · Zeitschrift für Gastroenterologie · 2015 · Heft 12
Bibliografische Angaben
Zusammenfassung
Background: PIK3CA, encoding the catalytic subunit p110α of class I phosphoinositide-3-kinase (PI3K), is mutated or overexpressed in many tumors, including hepatocellular carcinoma (HCC), where it is supposed to act as an oncogene. Here, we developed new mouse models to investigate the oncogenic effects of gain-of-function PIK3CA mutations and identified downstream PI3K signaling components in the mouse liver. Methods: PIK3CA mutants G1633A:E545K and A1340G:H1047R were overexpressed in the liver of 129/Sv-C57BL/6 mice via hydrodynamic gene delivery (HGD). Three, six, nine and twelve months after HGD, preneoplast…
