Comparison of different tag purification approaches to create GATA1-/GATA1 s-centered protein interaction networks

Introduction: Children with trisomy 21 (Down syndrome, DS) are predisposed to develop acute megakaryoblastic leukemia (DS-AMKL). Mutations in the transcription factor GATA1 and translation of the resulting truncated protein (GATA1 s) are both essential and sufficient to cause transient abnormal myelopoiesis (TAM) in synergy with trisomy 21. However, the molecular interplay between GATA1 s and trisomy 21 remains incompletely understood. Methods: To elucidate the GATA1/GATA1 s interactome, we optimized affinity purification (IP) approaches for tagged GATA1/GATA1 s followed by mass spectrometry (MS). To avoid cross…