Engineered precursor T cells from human umbilical cord blood using an alpharetroviral vector platform
J Hübner, SS Hoseini, JD Suerth, D Hoffmann, M Maluski, J Herbst, B Eiz-Vesper, M Heuser, A Schambach, MG Sauer · Klinische Pädiatrie · 2016 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Alpharetroviral vectors may reduce the risk of insertional mutagenesis. Methods: Cord blood-derived CD34+ cells were transduced and differentiated into preTs in vitro. Two promoter-envelope combinations were comparatively assessed. A chimeric antigen receptor (CAR) against CD123 along with the inducible suicide gene iCaspase 9 were used as genes of interest. Results: The sarcoma virus-derived promoter with a modified feline endogenous retrovirus envelope glycoprotein yielded in superior transduction and expression rates. Transgene positive cells did neither show proliferative impairment nor alteratio…
