Bacterial translocation into the mucus of crypts is associated with proximal colonic tumorigenesis in IL-10-/-xMSH2loxP/loxP Vill-cre (DKO) mice

Background: Intestinal dysbiosis is associated with inflammatory bowel disease and colorectal cancer. In humans, specifically right-sided tumors are associated with bacterial biofilms and invasive bacteria. Typically, Lynch syndrome patients have right-sided tumors, which appear mucinous with a high inflammatory infiltrate. Herein we cross-bred MSH2loxP/loxP Vill-cre mice into the IL-10-/- background to study the importance of inflammation and bacterial biofilms in Lynch syndrome tumorigenesis. Methods: IL-10-/-, MSH2loxP/loxP Vill-cre and IL-10-/-xMSH2loxP/loxP Vill-cre (DKO) mice were kept under both conventio…