Chemosensory cholinergic signaling network in the thymic medullary epithelium
A Soultanova, C Cen, K Fleck, G Krasteva-Christ, U Boehm, SM Wienhold, H Müller-Redetzky, M Witzenrath, W Kummer · Pneumologie · 2016 · Heft 07
Bibliografische Angaben
Zusammenfassung
Objective: A subset of medullary epithelial cells in the thymus (mTECs) was previously shown to be cholinergic and express components of bitter taste cascade. In this study we set out to further characterize these cells and elucidate their function. Methods: Immunohistochemistry, real-time RT-PCR and intracellular calcium measurements were conducted on thymi from ChAT- (choline acetyltransferase) and Chrna3-eGFP (nicotinic receptor subunit alpha3) reporter mice, mice expressing diphtheria toxin A driven by TRPM5 promoter (TRPM5: channel in taste transduction signaling), and wild-type mice with streptococcal pneu…
