Acidosis-driven aberrations of microRNAs in endometrial cancer in vitro
M Hirschfeld, D Weiß, M Jäger, S Mayer, J Asberger, C Nöthling, BN Trulley, G Gitsch, T Erbes · Geburtshilfe und Frauenheilkunde · 2016 · Heft 10
Bibliografische Angaben
Zusammenfassung
Purpose: MicroRNAs have been identified as crucial regulators of carcinogenic pathways. Micro environmental alterations such as hypoxia and acidosis frequently accompany tumor progression and metastasis. Thereto expression profiles of 24 different microRNAs, exhibiting oncogenic as well as tumor suppressor functions, were investigated under the influence of these two exogenic stimuli in endometrial cancer (EC) in vitro. Material and methods: Quantitative analyses of 24 specific microRNA specimen were performed by RNA isolation and subsequent realtime PCR from three established EC cell types. Therefore, relative…
