Immunological phenotype of freshly isolated human microvascular endothelial cells from human term placenta

Objectives: It is known that endothelial cells may play an important role in the pathogenesis in angiogenetic events as well as in disturbances of pregnancy like preeclampsia, intrauterine growth retardation. In order to examine pathophysiological mechanisms, particularly, in the endothelium of placenta, we isolated human placental microvascular endothelial cells . Methods: Fine minced specimens from fresh placenta cotyledons were washed three times in phosphate buffered saline, then incubated overnight in 1.2 units dispase II per ml Puck's solution at 4°C. Afterwards specimens were gently pressed by using syrin…