Impaired Hepatobiliary Lipid Metabolism in Liver Fatty Acid Binding Protein 1 (FABP1) Knockout Mice

Recent evidence from studies with sterol carrier protein 2 deficient and diosgenin fed mice support a role of FABP1 in biliary lipid secretion and gallstone formation. We explored whether alterations in biliary lipid secretion and metabolism occur in mice with FABP1 deficiency Male Fabp1 (-/-) and wild-type mice (n=6–9) received either a standard (SD) or a lithogenic diet (LD) for 4 weeks. Following collection of hepatic bile, livers were harvested for isolation of total RNA, homogenate and plasma membranes. Biliary lipids were analyzed by standard methods and biliary lipid secretion rates were calculated. Gene…