A novel possibility for gene transfer into the duct cells using non-replicating pseudorabies virus variants
M Kovács, Z Rakonczay, B Ördögh, T Takács, J Lonovics, A Varró, A Tóth, G Papp, M Gray, B Argent, Z Boldogkői, P Hegyi · Zeitschrift für Gastroenterologie · 2005 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Several types of viral vectors are currently in use for gene transfer, but there is no available method to transfect intact pancreatic ducts. Viral invasion of cells may lead to cytotoxicity, which may question the validity of any observations. Our aim was to establish a model system for transferring genes into the pancreatic duct cells, which did not lead to cell damage. Methods: The non-virulent Ka-RREp0lacgfp (KEG) genetically modified strain of pseudorabies virus (PRV) containing the gene for GFP was used in this study. Small intra/interlobular ducts were isolated from the pancreas of guinea pigs…
