Role of glutathione in the induction of hemoxygenase-1 enzyme in intestinal epithelial cells
Cs Varga, K Horváth, M Cavicchi, D Lamarque, J Delchier, J Kiss, B Whittle, F László · Zeitschrift für Gastroenterologie · 2005 · Heft 05
Bibliografische Angaben
Zusammenfassung
Hemoxygenase-1 (HO-1) enzyme plays a protective role in the mechanism of inflammatory bowel disease. The expression of HO-1 can be induced by a number of agents, e.g. by heavy metals and hem (FePP). The mechanism of HO-1 induction is not fully understood, however, the involvement of glutathione (GSH) is suspected. AIM: We investigated the interaction of GSH with HO-1 expression in a human epithelial cell line (DLD-1). Methods: The expression of HO-1 and the GSH level were determined by Western Blot and by the DTNB method, respectively. Cells were treated with the GSH promoter, N-acetylcisteine (NAC; 10 mM)) or w…
