Short-term ischaemia usually used for iscahemic preconditioning induces loss of dendritic integrity after long-term survival despite preserved neuronal cell density

Transient cerebral ischemia of only 5min duration causes selective and delayed neuronal death (DND) of hippocampal CA1 pyramidal cells. DND can be prevented by a short per se harmless preceding ischemic stimulus, a phenomenon called preconditioning. Little is known, however, about the structural long-term outcome after short-term ischemia. To probe for subtle dendritic alterations, we analyzed postischemic hippocampal protein expression of various dendritic marker proteins: (i) Synaptopodin, a 100 kDa protein, which is tightly associated with the structure and molecular function of spine apparatuses of hippocamp…