CareLit Fachartikel
3 D Reconstruction of Cervical Carcinoma Invasion Front using H&E/p16INK4a/CD3 Alternate Staining
J Einenkel, N Wentzensen, UD Braumann, JP Kuska, LC Horn · Geburtshilfe und Frauenheilkunde · 2008 · Heft 09
Dokument
968116
CareLit-ID
Jahr
2008
Publikation
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DOI
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Zusammenfassung
Background In this present study, we aimed to analyze the spatial organization of a cervical cancer (CX) and the relation of the tumor invasion front and the infiltration with CD3 positive T-cells. Methods 84 serial sections from CX were stained with H&E and immunohistochemically against the cervical p16 and the T-cell marker CD3. Sections were passed through an image processing chain in order to obtain a reconstructed tissue volume including both automatically delineated tumor and T-cell volume segments. For invasion front analysis mean curvature was used, which in turn was related to the respective local minim…
Schlagworte
cancer
cervix
front of invasion
inflammatory response
tumor biology
Geburtshilfe und Frauenheilkunde
