The adenoviral vector adsensor-p53t provides a highly selective tool for oncolytic virotherapy of tumors with activated telomerase and aberrations in p53-transcriptional activity

Oncolytic virotherapy is a promising and novel mean for treatment of solid tumors. A major challenge in virotherapy is tight restriction of viral replication to malignant cells combined with a large spectrum of potential target tumors. Aim of this study was the generation of a conditionally replicating adenovirus able to both recognize and to respond to activated telomerase and an aberrant transcriptional p53-status of the infected target cell by the onset of replication and lysis. We first developed a Gal4-binding hTert-promotor by flanking with Gal4-recognition sites. Luciferase-assays showed that the promoter…