CareLit Fachartikel
Glucosylcerebrosid accumulation in liver of bile acid beta glucosidase (Gba2) knockout mouse
Y Yildiz, H Matern, S Matern, T Sauerbruch, F Lammert, DW Russell · Zeitschrift für Gastroenterologie · 2007 · Heft 01
Dokument
971547
CareLit-ID
Jahr
2007
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
A novel microsomal bile acid beta glucosidase (GBA2) has been purified and characterized from human liver. The bile acid beta glucosidase hydrolyzes specifically bile acid 3-O-glucosides as endogenous compounds in liver. However, GBA2 is highly expressed in many tissues, including liver, brain, testes, bone marrow, and muscle. Since some of these tissues are not involved in bile acid metabolism, a Gba2 knockout (Gba2 -/-) mouse was generated to further characterize the function(s) of GBA2. Methods: Vector construction for Gba2 -/- generation, ES cell transformation, screening and blastocyst injections were carri…
Schlagworte
Gba2 knockout mouse
bile acid metabolism
glucosylcerebrosid accumulation. globozoospermia
Beta-Glucosidase
Chromatography
Blastocyst
Acrosome
Zeitschrift für Gastroenterologie
