Comparing cytochtome P450 activities of blood monocyte derived Neo-Hepatocytes and primary hepatocytes

The human body is constantly under assault by chemicals encountered in the environment. Altering their molecular structure to increase polarity is essential for the elimination from the body. Most bio-activation reactions are catalyzed by a group of hepatic microsomal monooxygenases. Its reaction specificity is defined by cytochrome P450 (CYP), a family of structurally related hemoproteins. Thus, CYPs are essential for the determination of pharmacologic and toxic effects, which are conventionally measured in primary human hepatocytes, whose availability is limited due to donor organ scarcity. This raises the dem…