A new dynamic culture system for in vitro maturation of fresh and frozen ovarian tissues in mice

Background: Reintroduction of malignant cells by ovarian tissue autotransplantation in cancer patients seeking fertility preservation is still a risk. Therefore, ovarian tissue cryopreservation followed by in vitro maturation could be a safer option. Objective: To establish a new dynamic culture system for in vitro maturation (IVM) of fresh & frozen ovarian tissue in mice. Materials and Methods: 72 ovaries were obtained from 36 female C57BL/6 mice 7–14 weeks old. 18 ovaries were frozen/thawed via slow freezing/rapid thawing protocol. 27 dynamic culture chambers (DCCs) were compared to 21 static culture chambers…