Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system
SA Hoffmann, M Lübberstedt, U Müller-Vieira, D Knobeloch, A Nüssler, JC Gerlach, K Zeilinger · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
Classical model systems of hepatotoxicity have profound limitations in adequately assessing drug toxicity: Results from animal experiments cannot necessarily be extrapolated to humans; primary human hepatocytes lose their specific metabolic functions within short time in conventional 2D cell culture. Based on a liver cell bioreactor for clinical use in an extracorporeal liver support system employing hollow fiber perfusion and internal oxygenation a down-scaled system (0.5ml cell volume) for in vitro studies was recently developed. This study aims to investigate the miniaturized bioreactor’s capacity for long-te…
