Targeted siRNA delivery to myeloid precursor cells

RNA interference (RNAi) provides a new way to target „undruggable sides“ of the body for several kind of diseases, e.g. leukaemia. Several fusion proteins are indispensable for the maintenance of leukaemic growth both in vitro and in vivo. Therefore, we introduced an antibody-conjugated immunoliposomes which deliver siRNAs to leukaemic cells and subsequently downregulated the targeted fusion gene of AML1/MTG8. Different liposomal formulations conjugated to an anti-CD33 single chain fragment (scFv CD33) were analyzed in vitro for their cytotoxicity, immune response, cell association, uptake and efficacy. In addit…