Non-conjugated bile acids induce ATP depletion, mitochondrial damage and inhibit the ion transport mechanisms in human colonic crypts
É Pallagi-Kunstár, K Farkas, Z Rakonczay, F Nagy, T Molnár, Z Szepes, V Venglovecz, Z Rázga, J Maléth, K Orbán, K Tóth, T Wittmann, P Hegyi · Zeitschrift für Gastroenterologie · 2012 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: Under pathophysiological conditions, such as short bowel syndrome, bile acids can reach the colon in high concentrations and can induce diarrhea. Our aim was to investigate whether impaired ion transport activities are involved in the pathomechanism of bile acid-induced diarrhea. Methods: Colonic biopsies were obtained from control patients (with negative colonoscopic findings) and from cholecystectomised/ileum-resected patients with/without diarrhea. Colonic crypts were isolated by collagenase digestion, and intracellular pH (pHi) and ATP levels (ATPi) were measured by microspectrofluorometry. Na+…
