Innate immune system modulated the expression of RANTES and the function of activated primary hepatic stellate cells

Background/Aims: Little is known how innate immune system modulate the expression of RANTES, thus progress the HCV-induced liver fibrosis. As Toll-like receptors (TLRs) and RIG-I like receptors (RLRs) are important in the regulation of innate and adaptive immune responses, we studied their role in the activation of HSC. Methods: Primary isolated murine and human HSC were stimulated with TLR1–9 or RIG-I ligands for 24h. The expression of cytokines and C-C Motif Chemokine receptor 5 (CCR5) was estimated by quantitative RT-PCR while chemokine (C-C motif) ligand 5 (RANTES) production was measured by ELISA. Functiona…