Chemotaxis and Transcription Factor Activation of Wild-Type and CCR6-Transfected RLE-6TN

Using a phage display approach we identified CCR6 as a putative receptor for CCL18. After stimulation with CCL18 the rat alveolar epithelial cell line RLE-6TN up-regulates CD90 expression and undergoes morphological changes implying EMT. However, these cells do not up-regulate alphaSMA expression by CCL18 stimulation. Analysis of these cells demonstrates that they express the previously described CCL18 receptor gpr30 but not CCR6. We therefore transfected RLE-6TN with a CCR6-eGFP construct. Here we present first data on differences of wild type RLE-6TN (wtRLE) and CCR6-eGFP transfected RLE-6TN (RLE*CCR6). FACS a…