Molecular characterization of lineage-specific long non-coding RNAs in hematopoiesis

Long intervening non-coding RNAs (lincRNAs) play an important role in the functional regulation of the genome. We aimed to extend present collections of hematopoietic mRNA expression profiles to a non-coding RNA HemAtlas by performing microarray expression analysis of long ncRNAs from differentiated blood cell lineages. Principal component analysis, hierarchical unsupervised clustering and Pearson's correlation confirmed the quality of our data. Applying integrated bioinformatics, we determined the top 50 specifically expressed lincRNAs for each blood cell lineage. Using genome databases, we designed primers for…