Generation and characterization of ganglidiximab for active immunotherapy of neuroblastoma

Introduction: Ganglidiomab is a murine IgG1 anti-idiotype antibody of anti-GD2 Ab ch14.18. We previously demonstrated an active GD2-specific humoral immune response against neuroblastoma (NB) after immunization of mice with ganglidiomab. To tailor this response to the murine variable regions in humans and to minimize nonspecific response against murine constant regions, we exchanged murine with human constant regions by Ab-engineering techniques. Methods and Results: Murine variable heavy (VH) and light chain (VL) cDNAs of ganglidiomab were identified, adapted by PCR and cloned into the pdHL7 vector in frame wit…